Analysis and interpretation of bulk RNA sequencing data with Bioconductor: This course shows how to use Bioconductor packages to analyse bulk RNA-seq data including how to import and annotate quantified data into R, exploratory analysis and quality control, differential expression analysis and gene set enrichment analysis.
Pre-requisites: Familiarity with R and Bioconductor.
Instructors: Estefania Mancini, Vasileios Panagiotis Lenis, Jacques Serizay
Dates: Monday and Tuesday, 15–16 September 2025
Venue: School of Medicine and Life Sciences, Universitat Pompeu Fabra (UPF), Barcelona
Room: 228 (except for Day 1 setup and welcome, which are in Room 232)
Coffee Break and Lunch:
- Day 1 (Monday): Served on floor -1
- Day 2 (Tuesday): Served in the lobby in front of the cafeteria
All participants are expected to follow the Bioconductor Code of Conduct.
Subject to change.
| Time | Session | Instructor(s) |
|---|---|---|
| 08:30–09:00 | Setup help (Room 232) | All |
| 09:00–09:15 | Welcome (Room 232) | Robert C & Maria |
| 09:15–10:30 | Introduction to RNA-seq | Vasilis |
| 10:30–11:00 | Coffee break | |
| 11:00–13:00 | RStudio Project and Experimental Data. | Estefania |
| 13:00–14:00 | Lunch | |
| 14:00–15:30 | Importing and annotating quantified data | Jacques |
| 15:30–15:45 | Comfort break | |
| 15:45–16:45 | Exploratory analysis and quality control | Vasilis |
| 16:45–17:00 | Wrap-up and Q&A | TBD |
| Time | Session | Instructor(s) |
|---|---|---|
| 08:30–09:00 | Helpdesk | All |
| 09:00–09:15 | Recap and Q&A | Jacques/Vasilis |
| 09:15–10:30 | Exploratory analysis (continued) | Jacques |
| 10:30–11:00 | Coffee break | |
| 11:00–13:00 | Differential expression analysis | Estefania/Vasilis |
| 13:00–14:00 | Lunch | |
| 14:00–15:30 | Gene set enrichment analysis | Jacques |
| 15:30–15:45 | Comfort break | |
| 15:45–16:45 | Gene set enrichment analysis (continued) | Jacques |
| 16:45–17:00 | Wrap-up and feedback | All |
Please ensure you have the following installed: